Blood/Plasma Partitioning
Assay System: 
  • Fresh blood from desired animal species.
  • Partitioning of test compound measured between red blood cells (RBC) and plasma.

Assay Conditions:

Test Compound Concentration       10 µM
Percent Organic Solvent in Incubation       1% acetonitrile
Incubation Time       60 minutes
pH       adjusted by 2-fold dilution of blood with PBS buffer prior to incubation
Temperature       37 °C
Positive Control       Chlorthalidone
Analysis       LC-MS/MS
Number of Repeats       3

Deliverable:
 RBC-to-Plasma ratio (KRBC/PL).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.