Cytotoxicity in Cultured HepG2 Cells
Assay System: 
  • Cultured monolayers of HepG2 cells.
  • Cytotoxicity of test compound measured by comparing cell viability of confluent HepG2 cells incubated with test compound against vehicle control.

Assay Conditions:

Test Compound Concentration           8 concentrations ranging from 300-0.14 µM
Percent Organic Solvent in Incubation           1% DMSO
Incubation Time           24 hours
Incubation Conditions           37°C, 5% CO2, and saturating humidity
pH           7.4
Positive Control           Chlorpromazine
Analysis           Luminescence
Number of Repeats           3

Deliverable:
 LC50 of test compound.

Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 30 mM DMSO stock solution.