Cytotoxicity in Hepatocyte Suspensions
Assay System: 
  • Cryopreserved hepatocytes in suspension.
  • Human hepatocytes are pooled from 10 donors (mixed gender).  All other species are male.
  • Cytotoxicity of test compound measured by comparing cell viability of hepatocytes incubated with test compound against vehicle control.

Assay Conditions:

Test Compound Concentration           8 concentrations ranging from 300-0.14 µM
Percent Organic Solvent in Incubation           1% DMSO
Incubation Time           4 hours
Incubation Conditions           7°C, 5% CO2, and saturating humidity
Positive Control           Tamoxifen
Negative Control           Acetaminophen
Analysis           Luminescence
Number of Repeats           3
Hepatocyte Concentration           106 viable cells/mL

Deliverable:
 LC50 of test compound.

Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 30 mM DMSO stock solution.