Cytotoxicity in Hepatocyte Suspensions
Cryopreserved hepatocytes in suspension.
Human hepatocytes are pooled from 10 donors (mixed gender). All other species are male.
Cytotoxicity of test compound measured by comparing cell viability of hepatocytes incubated with test compound against vehicle control.
Test Compound Concentration
8 concentrations ranging from 300-0.14 µM
Percent Organic Solvent in Incubation
7°C, 5% CO
, and saturating humidity
Number of Repeats
of test compound.
Test Compound Requirement:
2-3 mg dry powder or 100-200 µL of 30 mM DMSO stock solution.