Detection and Identification of Reactive Metabolites
Assay System: 
  • Liver microsomes or S9 fractions. 
  • LC-MS/MS detection of reactive metabolites of test compound using glutathione or KCN as trapping reagents.

Assay Conditions:

Test Compound Concentration           50 µM
Incubation Time           3 hours
pH           7.4
Temperature           37 °C
Positive Control           Clozapine
Analysis           LC-MS/MS
Number of Repeats           2

Molecular weights and relative abundance of detected conjugates of test compound with glutathione or KCN. Tentative structures of conjugates determined using MS/MS analysis are available upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.