Detection and Identification of Reactive Metabolites
Liver microsomes or S9 fractions.
LC-MS/MS detection of reactive metabolites of test compound using glutathione or KCN as trapping reagents.
Test Compound Concentration
Number of Repeats
Deliverable: Molecular weights and relative abundance of detected conjugates of test compound with glutathione or KCN. Tentative structures of conjugates determined using MS/MS analysis are available upon request.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.