Cytochrome P450 Inhibition, Fluorescence Detection (Single Concentration)
Assay System: 
  • Individual human recombinant CYP isoforms expressed in baculovirus-infected insect cells.
  • Inhibition potential of test compound against recombinant CYP isoforms measured at a single compound concentration.

Assay Conditions:

Test Compound Concentration   10 µM (other concentrations can be tested upon request)
Percent Organic Solvent in Incubation   0.1% acetonitrile
CYP Isoforms Available for Testing   CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4
Incubation Time   15-45 minutes (depending on CYP isoform)
pH   7.4
Temperature   37°C
Positive Control   Furafylline (CYP1A2), Tranylcypromine (CYP2B6, CYP2C19), Quercetin (CYP2C8), Sulfaphenazole (CYP2C9), Quinidine (CYP2D6), Ketoconazole (CYP3A4)
Analysis   Fluorescence Detection
Number of Repeats   2

Deliverable: Percent inhibition of CYP isoforms by test compound at 10 µM.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM MeCN stock solution.