Cytochrome P450 Inhibition, Fluorescence Detection (Single Concentration)
Individual human recombinant CYP isoforms expressed in baculovirus-infected insect cells.
Inhibition potential of test compound against recombinant CYP isoforms measured at a single compound concentration.
Test Compound Concentration
10 µM (other concentrations can be tested upon request)
Percent Organic Solvent in Incubation
CYP Isoforms Available for Testing
CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4
15-45 minutes (depending on CYP isoform)
Furafylline (CYP1A2), Tranylcypromine (CYP2B6, CYP2C19), Quercetin (CYP2C8), Sulfaphenazole (CYP2C9), Quinidine (CYP2D6), Ketoconazole (CYP3A4)
Number of Repeats
Percent inhibition of CYP isoforms by test compound at 10 µM.
Test Compound Requirement:
mg dry powder or 100-200 µL of 10 mM MeCN stock solution.