In Vitro and In Vivo Metabolite Profiling and Identification
In vitro: Liver microsomes, S9 fractions, or hepatocytes.
In vivo: Biological fluids (e.g. plasma, urine) or tissues (e.g. brain, liver) from animal studies.
Identification of metabolites of test compound generated in Phase I and/or Phase II metabolic reactions.
Determination of tentative structures of metabolites using advanced LC-MS/MS analysis.
Test Compound Concentration
Number of Repeats
Tier 1 analysis: A list of identified metabolites, including corresponding molecular masses and relative abundance of metabolites estimated from integration of peak areas in HPLC-UV/LC-MS/MS chromatograms.
Tier 2 analysis: Tier 1 analysis results, plus MS/MS spectra of identified metabolites, suggested metabolite structures, and possible metabolic pathways of test compound.
Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.