Inhibition potential of test compound measured upon incubation with human liver microsomes at different compound concentrations. Reversible inhibition is determined by monitoring activity of individual CYP enzymes using isoform-specific probe substrates with LC-MS/MS detection.
Test Compound Concentration
Eight concentrations ranging from 0.05 µM to 100 µM
Percent Organic Solvent in Incubation
CYP Isoforms Available for Testing
CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4
10-20 minutes (depending on CYP isoform)
Number of Repeats
Deliverable: IC50 of test compound and positive control compound.
Test Compound Requirement: 1-2mg dry powder or 100-200 µL of 10 mM acetonitrile stock solution.