N-Acetylation Using Human Recombinant N-Acetyltransferases or Liver Cytosol
Assay System: 
  • Mammalian liver cytosol or major human recombinant N-acetyltransferases.
  • Formation of N-acetylated metabolites of test compound measured upon incubation with liver cytosol or recombinant N-acetyltransferases.
  • Position of acetylation in the test compound can be elucidated upon request.

Assay Conditions:

Test Compound Concentration           5 µM
Percent Organic Solvent in Incubation           1% acetonitrile
Incubation Time           30 minutes
pH           7.4
Temperature           37 °C
Positive Control           Sulfamethazine
Analysis           LC-MS/MS
Number of Repeats           3

Deliverable: 
Formation of N-acetylated metabolites (% of parent) and determination of acetylation site (optional).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.