PAMPA (Parallel Artificial Membrane Permeability Assay)
Assay System: 
  • Lipid-infused artificial membrane.
  • Passive transport of test compound measured through permeability across a phospholipid membrane.

Assay Conditions:

Test Compound Concentration           100 µM
Percent Organic Solvent in Incubation           1% DMSO
Incubation Time           5 hours
pH           7.4
Temperature           20 °C
Positive Control           Verapamil
Analysis           UV Absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats           2

Deliverable: Passive permeability (Pe), mass retention (R)

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.