CYP Reaction Phenotyping (Recombinant CYP Isoforms)
Assay System: 
  • Individually expressed recombinant human CYPs (rhCYPs).
  • Human CYP isoforms responsible for metabolism determined by measuring disappearance of test compound in incubations with individual rhCYPs.
Test Compound Concentration           1 µM
Incubation Time           30 minutes
pH           7.4
Temperature           37°C
Analysis           LC-MS/MS
Number of Repeats           2

Study Report:
 Percent of test compound remaining after incubation with individual rhCYPs.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.