Individual human recombinant CYP isoforms expressed in baculovirus-infected insect cells.
Inhibition potential against recombinant CYP isoforms (IC50) measured with and without pre-incubation in the presence of different concentrations of test compound by monitoring activity of individual CYP enzymes using isoform-specific probe substrates with fluorescence detection.
Time-dependent inhibition (IC50 shift) is determined by comparing IC50 values determined with and without pre-incubation.
Test Compound Concentration
Eight concentrations ranging from 0.05 µM to 100µM