Aqueous Solubility, Shake Flask
Assay System:
  • Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
  • Solubility measured by incubating test compound in PBS for 24 h, followed by determination of dissolved concentration against a calibration curve.

Assay Conditions:

Test Compound Concentration           100 µM
Percent Organic Solvent in Incubation           No organic solvent added
Incubation Time           24 hours
pH           7.4 or as requested
Temperature           Ambient
Positive Control           Verapamil and Tamoxifen
Analysis           UV/VIS absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats           2

Deliverable:
Solubility of test compound (µM).

Test Compound Requirement: 1-2 mg dry powder.