Aqueous Solubility, Kinetic
Assay System:
  • Phosphate Buffer Saline pH 7.4 (PBS); other aqueous systems available upon request.
  • Kinetic aqueous solubility measured by incubating test compound in PBS for 1 h, followed by determination of dissolved concentration against a calibration curve.

Assay Conditions:

Test Compound Concentration           100 µM
Percent Organic Solvent in Incubation           1% DMSO
Incubation Time           1 hours
pH           7.4 or as requested
Temperature           Ambient
Positive Control           Verapamil and Tamoxifen
Analysis           UV Absorbance or LC-MS (LC-MS/MS if necessary)
Number of Repeats           2

Deliverable:
Solubility of test compound (µM).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.