Chemical Stability
Assay System:
  • Standard test systems include Phosphate Buffered Saline (PBS) pH 7.4, Simulated Gastric Fluid (SGF), and Simulated Intestinal Fluid (SIF).
  • Additional test systems available upon request.
  • Stability determined by measuring percent remaining of test compound at multiple time points during incubation in selected media.

Assay Conditions:

Test Compound Concentration           10 µM
Percent Organic Solvent in Incubation           1% DMSO
Incubation Time (PBS)           0, 2, 4, 6, 8, 24, 48, 72 hours
Incubation Time (SGF, SIF)           0, 15, 30, 60, and 90 minutes
pH           7.4 (PBS), 1.2 (SGF), 5.0 (fed-state SIF), 6.5 (fasted-state SIF)
Temperature           37°C
Positive Control           Verapamil and Tamoxifen
Analysis           LC-MS (LC-MS/MS if necessary)
Number of Repeats           2

Deliverable:
Half-life of test compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.