CYP Induction in Cryopreserved Human Hepatocytes
Plated cryopreserved human hepatocytes from 3 individual donors.
Induction of CYP1A2, CYP2B6 and CYP3A4 by test compound measured by comparing enzyme activity of hepatocytes treated with test compound with vehicle control.
Test Compound Concentration
30 µM, 3 µM, and 0.3 µM
Percent Organic Solvent - Inducer Treatment
Percent Organic Solvent - Substrate Incubation
Inducer Treatment Time
Substrate Incubation Time
Treatment and Incubation Conditions
37 °C, 5% CO
, and saturating humidity
Omeprazole (1A2), Phenobarbital (2B6), and Rifampicin (3A4)
Number of Repeats
Mean fold induction of test compound and positive control compound.
Test Compound Requirement:
2-3 mg dry powder or 100-200 µL of 30 mM DMSO stock solution.