CYP Reaction Phenotyping (Liver Microsomes)
Assay System:
  • Human liver microsomes.
  • Human CYP isoforms responsible for metabolism of test compound determined by measuring metabolism suppression in the presence of CYP isoform-selective inhibitors.

Assay Conditions:

Test Compound Concentration           1 µM
Incubation Time           30 minutes
pH           7.4
Temperature           37 °C
Positive Control           CYP isoform-selective probe substrates
Analysis           LC-MS/MS
Number of Repeats           2

Study Report:
Percent of metabolism of test compound suppressed by CYP isoform-selective inhibitors.

Test Compound Requirement: 2-3 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.