Metabolic Stability in Liver Microsomes (Single Time Point)
Assay System: 
  • Mammalian liver microsomes.
  • Metabolic clearance determined by measuring percent remaining of test compound at a single time point after incubation with liver microsomes.

Assay Conditions:

Test Compound Concentration           1 µM
Percent Organic Solvent in Incubation           0.5% acetonitrile
Incubation Time           0 and 30 minutes
pH           7.4
Temperature           37 °C
Positive Control           Testosterone
Analysis           LC-MS (LC-MS/MS if necessary)
Number of Repeats           2

Percent remaining of test compound after 30 min incubation with liver microsomes. Incubation time can be adjusted as needed, upon request.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.