Metabolic Clearance in Liver Microsomes
Mammalian liver microsomes.
Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with liver microsomes.
Test Compound Concentration
Microsomal Protein Concentration
Percent Organic Solvent in Incubation
0, 5, 10, 15, 30, and 45 minutes
LC-MS (LC-MS/MS if necessary)
Number of Repeats
Intrinsic clearance, t
, and predicted blood extraction ratio (E
Test Compound Requirement:
1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.