Metabolic Clearance in Liver Microsomes
Assay System:
  • Mammalian liver microsomes.
  • Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with liver microsomes.

Assay Conditions:

Test Compound Concentration           1 µM
Microsomal Protein Concentration           1 mg/mL
Percent Organic Solvent in Incubation           0.5% acetonitrile
Incubation Time           0, 5, 10, 15, 30, and 45 minutes
pH           7.4
Temperature           37 °C
Positive Control           Testosterone
Analysis           LC-MS (LC-MS/MS if necessary)
Number of Repeats           2

Intrinsic clearance, t1/2, and predicted blood extraction ratio (EH).

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.