Metabolic Clearance in Liver S9 Fractions
Mammalian liver S9 fractions.
Human S9 fractions are pooled from 10 donors (mixed gender). All other species are male.
Metabolic clearance determined by measuring percent remaining of test compound at multiple time points during incubation with liver S9 fractions.
Test Compound Concentration
S9 Protein Concentration
NADPH, UDPGA, and PAPS
Percent Organic Solvent in Incubation
0, 5, 10, 15, 30, and 45 minutes
Testosterone and 7-hydroxycoumarin
LC-MS (LC-MS/MS if necessary)
Number of Repeats
Intrinsic clearance, t
, and predicted blood extraction ratio (E
Test Compound Requirement:
1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.