Protein Binding, Equilibrium Dialysis
Assay System:
  • Plasma, brain tissue, or liver microsomes (other tissues and biological fractions available upon request).
  • Protein binding of test compound measured using Rapid Equilibrium Dialysis (RED) device.

Assay Conditions:

Test Compound Concentration           1 µM
Percent Organic Solvent in Incubation           0.5% acetonitrile
Incubation Time           4 hours
pH           7.4, adjusted prior to incubation
Temperature           37 °C
Positive Control           Propranolol
Analysis           LC-MS/MS
Number of Repeats           3
Brain Homogenate           Prepared in 4 volumes of phosphate-buffered saline (PBS)

Deliverable: Percent bound of test compound and positive control compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.