Stability in Plasma
Assay System:
  • Blood plasma from desired species.
  • Stability determined by measuring percent remaining of test compound at multiple time points during incubation in plasma.

Assay Conditions:

Test Compound Concentration           10 µM
Percent Organic Solvent in Incubation           0.5% acetonitrile
Incubation Time           0, 15, 30, 60, and 90 minutes
pH           7.4
Temperature           37 °C
Positive Control           Propantheline and/or Enalapril
Analysis           LC-MS (LC-MS/MS if necessary)
Number of Repeats           2

Deliverable:
Half-life of test compound.

Test Compound Requirement: 1-2 mg dry powder or 100-200 µL of 10 mM DMSO stock solution.